FastQCFastQC Report
Thu 26 May 2016
ERR488999_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488999_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18550238
Sequences flagged as poor quality0
Sequence length75
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT464750.25053586913547954No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT352300.18991670079920267No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233810.1260415095482872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG202600.026.9803582
GTACCTG216450.025.823231
ACCTGGG252550.021.2581833
GAGTACT783450.021.11351812
AGTACTT802300.020.756613
GTACTTT812250.020.11066214
GTACATG1109600.019.6973921
AGAGTAC1465200.019.1682211
TACATGG1142950.018.8004912
ACATGGG1141050.018.3205013
ACTTTTT931450.018.05857516
TACTTTT922850.017.74723815
GTATAGG63800.017.2940561
TTAGTCG24350.017.14426839
CATGGGG525400.016.66034
GTGTAGC78100.016.4304581
TATCACG10850.016.2515852
CATGGGT172700.016.2344534
TACCCGG25750.016.1123662
TATAACG15350.015.9920922
Produced by FastQC (version 0.11.5)