FastQCFastQC Report
Thu 26 May 2016
ERR488999_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488999_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18550238
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440750.2375980297395645No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT369030.19893545301143847No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256560.13830550314233164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG203950.025.5097472
GTACCTG222000.024.119761
ACCTGGG250250.020.2248153
GAGTACT802850.019.66278612
AGTACTT819950.019.52225113
TAGATCG5800.019.0340255
GTACTTT830950.018.79052514
GTACATG1116100.018.0118851
AGAGTAC1521050.017.636211
TTAGTCG25800.017.38264739
TACATGG1147550.017.2270742
ACATGGG1145450.016.8430043
ACTTTTT941050.016.80471816
TACTTTT940900.016.6130715
CATGGGT173300.015.9658294
GTGTAGC83950.015.8323781
CGCGCGA2001.1444881E-515.52404124
TAGTCGG29150.015.38498440
CATGGGG528100.014.7118694
ATACGTA46700.014.55263425
Produced by FastQC (version 0.11.5)