FastQCFastQC Report
Thu 26 May 2016
ERR488998_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488998_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17520328
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175270.1000380814788399No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG238100.029.1790662
GTACCTG242500.029.124191
GTATCAA734350.026.398691
GGTATCA461250.025.6898061
ACCTGGG278950.024.1972143
TATCAAC911450.021.1563512
TCAACGC915050.020.9894454
ATCAACG916950.020.9257473
CAACGCA930400.020.6407875
AACGCAG960300.020.1191436
GAGTACT499600.020.098812
AGTACTT510850.019.54242913
AGAGTAC932600.019.34854711
GTACATG746350.019.0601371
TACATGG745150.018.6798342
CCTGGGG234400.018.3642624
GTACTTT543700.018.33516314
TACACGG32000.018.155582
ACGCAGA1074100.017.9005417
CGCAGAG1096550.017.5293398
Produced by FastQC (version 0.11.5)