FastQCFastQC Report
Thu 26 May 2016
ERR488998_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488998_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17520328
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG246150.028.4429111
TACCTGG242000.028.3552722
GTATCAA739200.025.5941941
GGTATCA471800.024.8497331
ACCTGGG277800.023.8690763
TATCAAC924000.020.3637432
TCAACGC925000.020.3193474
ATCAACG927300.020.254073
CAACGCA941050.019.9950775
GAGTACT508050.019.86843712
AACGCAG972700.019.429156
TATCACG13350.019.3818152
AGTACTT515300.019.38163813
AGAGTAC961550.018.68539811
GTACATG749300.018.3500351
GTACTTT542500.018.34628514
TACATGG750750.018.0091972
CCTGGGG227950.017.9195274
ACGCAGA1096400.017.2079777
ACATGGG770150.016.9418433
Produced by FastQC (version 0.11.5)