FastQCFastQC Report
Thu 26 May 2016
ERR488997_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488997_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19325141
Sequences flagged as poor quality0
Sequence length75
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219400.11353086634658965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG243600.023.6147822
GTACCTG248750.023.5603621
ACCTGGG271550.020.5826423
GAGTACT554550.019.52484112
AGTACTT568150.018.94891413
GTACTTT578900.018.54579714
TAGATCG12050.018.3573975
GTACATG848050.018.2395821
AGAGTAC1076400.018.0217211
TACATGG860300.017.7092842
TACTTTT628800.017.1325815
ACATGGG866950.017.0444473
ACTTTTT667950.016.55053116
GTACCCG32900.015.4579931
CATGGGG532100.014.9329934
GTATAGG41850.014.7148631
CCTGGGG248800.014.5583464
CATGGGT140100.014.5057544
TAGCCCT83950.014.4506564
TACCCGG32700.014.3780182
Produced by FastQC (version 0.11.5)