FastQCFastQC Report
Thu 26 May 2016
ERR488997_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488997_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19325141
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA1030370.5331759287034439No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA239780.12407671436912154No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217240.11241315134518294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG241550.022.8478091
TACCTGG240650.022.5907942
ACCTGGG268600.019.483233
TAGATCG13600.019.0233945
GAGTACT570650.018.76824612
AGTACTT579400.018.47290613
GTACTTT591550.018.01768314
GTACATG857750.017.5084551
TACCCGG30450.017.3326632
AGAGTAC1110700.017.32262211
TACATGG874200.016.9359682
TACTTTT641000.016.61139315
ACATGGG872650.016.3185273
ACTTTTT673800.016.03824416
GTACCCG34850.015.8758121
CATGGGG526450.014.402784
GAGTACG25250.014.2426461
CCTGGGG238550.013.7812694
GTGTAGC99450.013.3867351
CTTTTTT831300.013.0120517
Produced by FastQC (version 0.11.5)