FastQCFastQC Report
Thu 26 May 2016
ERR488996_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488996_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12134985
Sequences flagged as poor quality0
Sequence length75
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197370.16264544208336476No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145370.11979413241961157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG266050.040.7841031
TACCTGG263600.040.593532
ACCTGGG284500.036.8636973
CCTGGGG225650.030.2830054
TATAACG10900.030.1327442
ATAACGC13250.025.305883
GGTATCA372750.023.8000831
GTATCAA554450.023.4391211
GTACCCG27700.022.9835011
TACACGG22450.022.330232
TAACGCA16600.020.8218614
TACCCGG29150.020.5186652
GTACACG27450.020.293721
CTGGGGG125100.020.0312635
TATCAAC661800.019.4024542
TATCACG10800.019.207422
ATCAACG668950.019.1813833
TCAACGC669250.019.1711064
GAGTACT398450.018.95321312
GTACATG410250.018.9425851
Produced by FastQC (version 0.11.5)