FastQCFastQC Report
Thu 26 May 2016
ERR488996_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488996_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12134985
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193630.15956344404216405No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151990.12524943376526632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG269650.040.7464072
GTACCTG275050.040.1269951
ACCTGGG285450.037.3322563
CCTGGGG217100.030.6042774
TATAACG11900.028.6991142
GGTATCA396250.024.6510011
GTACCCG28300.023.214141
TAACGCA15250.023.0736314
ATAACGC15400.023.0734673
GTATCAA573900.022.2378711
TACCCGG28950.021.8063412
TATCACG9050.021.727342
CTGGGGG115950.020.5880685
TACACGG23250.020.4755882
TACCGGG17200.019.0535282
GTACACG30600.018.5313821
GAGTACT419650.018.36331712
TATCAAC691350.018.3175222
TCAACGC692700.018.2621274
ATCAACG691900.018.2388213
Produced by FastQC (version 0.11.5)