FastQCFastQC Report
Thu 26 May 2016
ERR488995_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488995_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9390801
Sequences flagged as poor quality0
Sequence length75
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260460.2773565322063581No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181090.1928376503772149No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG168000.17889847735033465No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA167610.17848317731362853No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130820.1393065405176832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG77450.026.8246822
GTACCTG92000.023.0379091
TATCACG5800.022.6107562
GAGTACT518000.022.10160612
AGTACTT522500.021.95203813
TATAACG7050.021.0493582
GTACTTT550650.020.63678614
ACCTGGG101100.019.9664573
GTACATG788600.019.8729171
TACATGG788200.019.6724972
AGAGTAC986850.019.4107111
ACTTTTT606200.019.0554716
ACATGGG795950.018.8495083
TACTTTT605500.018.76741215
GAGTACG18600.018.7443751
CATTCCG17300.018.736079
CATGGGG376750.018.4557674
ATAACGC8800.018.4294133
CATGGGT84650.017.6103134
GTACACG14600.016.7868041
Produced by FastQC (version 0.11.5)