FastQCFastQC Report
Thu 26 May 2016
ERR488995_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488995_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9390801
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA2049432.1823803954529546No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA488480.5201686203338778No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG461110.49102307673221907No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC332170.35371849536583727No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254290.2707862726512893No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187250.19939726121339382No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA162640.17319076402534778No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137870.14681388733506331No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG83900.024.9234432
GAGTACG16950.023.460531
GTACCTG97700.021.6957951
TATAACG7450.021.3058552
GAGTACT541300.020.87223212
AGTACTT546000.020.65476213
CATTCCG17400.019.8257779
GTACTTT571000.019.62356414
TACGCAG18150.019.3912034
ACCTGGG106700.018.9825633
GTACATG766550.018.7791351
AGAGTAC1016450.018.50059911
TACATGG768400.018.4835512
ATAACGC8900.018.2217083
ACTTTTT621700.018.16168616
CGGTGTT18450.018.13732714
TACTTTT624500.017.95320315
ACATGGG768850.017.7494963
TACCCGG8400.017.253112
GTACGCA20600.017.2524683
Produced by FastQC (version 0.11.5)