FastQCFastQC Report
Thu 26 May 2016
ERR488994_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488994_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9425233
Sequences flagged as poor quality0
Sequence length75
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231850.24598861375628592No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210930.2237928759957446No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163210.17316282791099172No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118140.12534438140680448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG254700.045.77662
GTACCTG260000.045.2579841
ACCTGGG265300.042.9302943
CCTGGGG198250.036.8784074
CTGGGGG117350.029.969915
TATAACG10600.029.3489632
TACACGG21700.027.5576692
TAACGCA11750.026.4651474
GTACACG25100.025.860491
TACCCGG19600.025.5721972
TATCACG8000.025.4928342
ATAACGC13250.023.4714853
TGGGGGG201600.022.5714386
GTATCAA565200.022.3178141
TACCGGG14350.022.1611372
GTACCCG24800.021.6944621
GTACATG404800.021.3428921
GTACCGG18300.020.8646181
TACATGG414000.020.522852
GGTATCA387850.020.3783071
Produced by FastQC (version 0.11.5)