FastQCFastQC Report
Thu 26 May 2016
ERR488994_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488994_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9425233
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236570.2509964475148784No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202550.21490184911078591No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170360.18074884726987653No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121470.12887745056276062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG263800.045.2901882
GTACCTG268950.044.7911951
ACCTGGG273000.042.6012653
CCTGGGG199350.037.3107154
TATAACG11450.033.747292
CTGGGGG110200.031.5558595
TACCCGG20850.031.2739092
TAACGCA13050.029.8723244
ATAACGC14150.028.7708023
TACACGG23500.026.2791652
GTACACG24600.025.8698231
TATCACG6700.025.7467022
GTACCCG28400.024.9658451
TGGGGGG181250.023.4685426
TACCGGG13850.022.170052
GTATCAA571000.022.0241171
GTACATG395650.021.032711
GGTATCA393900.020.8363911
TACATGG406050.020.2304842
GTACGCT7700.020.1602179
Produced by FastQC (version 0.11.5)