FastQCFastQC Report
Thu 26 May 2016
ERR488993_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488993_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16080951
Sequences flagged as poor quality0
Sequence length75
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA4015952.4973336464988916No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA1230050.7649112294415921No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA895900.5571187922903317No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC669390.41626269491151363No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG338940.21077111670820958No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG328350.2041856852868963No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161280.10029257598011461No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG46700.024.3291951
TACCTGG175050.023.1334862
GTACCTG183300.022.4198781
TACGCAG47450.021.8250224
TATAACG6950.020.862662
GCTTATG50800.020.394181
ACCTGGG201850.019.2557983
ACGCAGT53950.019.2540935
GTACATG706750.019.0273741
GAGTACT436750.018.90916412
AGTACTT438100.018.70222913
TACATGG715500.018.5616992
GTACTTT452050.018.11882814
TACTTTT467550.017.51315115
ACATGGG731200.017.4858473
AGAGTAC886350.017.36237111
ACTTTTT498500.016.81217616
CTTATGT60450.016.5046652
GTATATA33800.015.5301491
CATGGGG417550.015.4845624
Produced by FastQC (version 0.11.5)