FastQCFastQC Report
Thu 26 May 2016
ERR488993_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488993_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16080951
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA3962702.4642199332614094No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC675940.42033583710316635No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG456850.2840938946956557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG49350.024.5936261
TACGCAG47200.023.2451254
TATAACG6700.022.6593572
TACCTGG173950.021.7199782
GTACCTG180900.021.3700661
GCTTATG52900.020.5900551
ACGCAGT51900.020.5417045
GAGTACT441200.018.74251712
AGTACTT442550.018.48272513
ACCTGGG198550.018.1764283
GTACTTT455550.017.87955514
GTACATG704350.017.786171
TACATGG713700.017.225422
TACTTTT475150.017.13433315
CTTATGT63000.016.9233992
TATGCGT5750.016.7984567
AGAGTAC905800.016.49876811
ACATGGG725850.016.356283
ACTTTTT504200.016.28396216
GTACGCA73500.015.5377243
Produced by FastQC (version 0.11.5)