FastQCFastQC Report
Thu 26 May 2016
ERR488992_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488992_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15374458
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA590250.38391597284275No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA170540.11092423550800945No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165570.10769160122587737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG235300.035.16881
TACCTGG237250.034.380722
ACCTGGG269850.029.3421653
CCTGGGG217300.023.8005734
TATAACG12500.023.5226522
TATCACG11350.021.9439092
GAGTACT455300.019.46689412
ATAACGC16350.019.0411663
TAACGCA15450.019.0325244
AGAGTAC711650.018.86310811
AGTACTT471650.018.71313713
GTACATG472950.018.5396541
GTACACG32150.018.0381051
TACATGG488300.017.724662
GTACTTT505300.017.41236914
GTACCCG25000.017.3630281
TACACGG30000.016.834842
ACATGGG495600.016.737353
TACTTTT545350.016.12859515
TACCCGG26350.015.7535342
Produced by FastQC (version 0.11.5)