FastQCFastQC Report
Thu 26 May 2016
ERR488992_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488992_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15374458
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA616790.40117836999522194No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160820.10460206141901067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG237000.034.6177371
TACCTGG236950.033.9856762
ACCTGGG273250.028.662723
TATAACG12350.025.143592
CCTGGGG221000.022.5431884
TAACGCA15050.022.0077254
ATAACGC16300.021.8022523
GTACCCG26550.018.759891
TACCCGG25200.018.757362
GAGTACT455100.018.7394412
AGTACTT465150.018.2307213
AGAGTAC727900.017.94419111
TACACGG29450.017.6906342
TATCACG11450.017.4772972
GTACATG483200.017.2656381
GTACTTT497550.016.95341314
GTACACG34150.016.9144571
TACATGG496050.016.4635892
TACTTTT535650.015.80432615
ACATGGG500800.015.6529333
Produced by FastQC (version 0.11.5)