FastQCFastQC Report
Thu 26 May 2016
ERR488991_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488991_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19113183
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT273230.14295368803825087No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195470.10226972660702302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG224700.024.6174031
TACCTGG222150.024.0435582
ACCTGGG260000.019.9683933
GAGTACT589100.019.86001212
AGTACTT590700.019.7482113
GTACTTT612050.019.0335314
GTACATG778150.018.453821
AGAGTAC1051000.018.3906611
TACATGG767600.018.222482
TATAACG13100.017.6815412
TACTTTT665050.017.52530715
ACATGGG798300.017.0599063
ACTTTTT702950.016.92262816
GAGTACG27150.016.4367791
GTGTAGC90950.016.4315261
CATGGGG489400.015.5090884
CCTGGGG222250.015.3028124
GTACCCG32150.014.5261211
TACACGG28250.014.0733012
GTACACG37700.014.0393491
Produced by FastQC (version 0.11.5)