FastQCFastQC Report
Thu 26 May 2016
ERR488991_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488991_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19113183
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA1054580.5517552989473287No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270220.14137885876988673No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG235440.12318199433343992No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206550.108066772551699No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG223950.023.8392811
TACCTGG220500.023.7019462
ACCTGGG267400.019.0421643
GAGTACT598150.018.47662512
AGTACTT604850.018.19211613
GTACTTT619750.017.67125514
GTACATG791050.017.5238321
TACATGG779000.017.3502982
AGAGTAC1081700.017.27388211
TATAACG11850.017.1756692
TACTTTT667300.016.42751515
ACATGGG800500.016.3115753
GAGTACG27550.016.190681
ACTTTTT698450.015.84796316
GTGTAGC93900.015.2083261
CATGGGG488650.015.1432284
GTATAGC48750.014.9659681
CCTGGGG220450.014.6628554
GTACCCG35700.014.23790551
GTATAGG36800.013.7183541
Produced by FastQC (version 0.11.5)