FastQCFastQC Report
Thu 26 May 2016
ERR488990_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488990_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11107352
Sequences flagged as poor quality0
Sequence length75
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA316210.28468531473568137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG125250.022.830071
TACCTGG124650.022.3446962
GAGTACT292600.020.34858512
AGTACTT301950.019.61661513
ACCTGGG136200.019.5562923
AGAGTAC573400.019.08382411
GAGTACG18950.018.839051
GTACTTT317200.018.6514314
GTACATG468750.017.8273581
TACTTTT341200.017.36443315
TACATGG480600.017.0834392
TACGCAG19400.016.9623854
ACTTTTT356250.016.92808516
TATCACG7250.016.7240222
ACATGGG501450.015.9420963
TATAACG6350.015.8210382
ACGCAGT21900.014.3919185
GTACACG23100.014.2541931
CATGGGG298000.014.1926824
CTTTTTT450050.013.48340417
Produced by FastQC (version 0.11.5)