FastQCFastQC Report
Thu 26 May 2016
ERR488990_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488990_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11107352
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA1076970.9696010354223041No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA315950.2844512355420086No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC196540.17694586432481837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG128450.023.1533372
GTACCTG131700.022.8238281
GAGTACG18500.020.941161
ACCTGGG140300.020.1403353
GAGTACT310350.018.99737512
AGTACTT317350.018.65443413
AGAGTAC588650.018.04479611
GTACTTT329000.017.83658614
GTACATG472400.017.5001771
TATAACG6550.017.3825342
TACATGG487200.016.7976252
GTATAGG16350.016.7133621
TACGCAG20650.016.5409154
TACTTTT358400.016.35351415
ACTTTTT365550.016.11858216
ACATGGG502900.015.6969353
CCTGGGG123600.014.4316654
ATAACGC11050.014.3626983
ACGCAGT23550.014.3575235
GTACACG22850.014.2297121
Produced by FastQC (version 0.11.5)