FastQCFastQC Report
Thu 26 May 2016
ERR488989_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488989_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14274404
Sequences flagged as poor quality0
Sequence length75
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248550.17412285654798618No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179540.1257775806261333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG256900.038.6400761
TACCTGG255800.038.538012
ACCTGGG285800.033.7618063
CCTGGGG228500.026.8718224
ATAACGC16400.025.9292133
TATAACG16700.024.6392
TAACGCA18800.022.2519424
GTACCCG24250.021.822271
GTATCAA747150.021.4890671
GTACACG30350.020.7411521
TACACGG27100.020.6698992
GGTATCA525200.020.0860861
GAGTACT502750.019.1871912
AGAGTAC762750.018.94628711
AGTACTT514350.018.66910713
GTACATG467550.018.5236451
TACCCGG27750.018.3166892
TATCAAC880700.018.0798262
TACATGG472750.017.8683532
TCAACGC888600.017.8585744
Produced by FastQC (version 0.11.5)