FastQCFastQC Report
Thu 26 May 2016
ERR488989_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488989_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14274404
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233140.16332730949747534No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186810.13087061288163063No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143710.10067670776307018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG257400.037.294561
TACCTGG255550.037.0323262
ACCTGGG280000.033.0593073
CCTGGGG223100.026.7371964
ATAACGC15750.023.876853
TATAACG15350.023.1504762
GTACCCG25050.023.0704631
TATCACG11550.021.208382
TACCCGG26200.021.0692862
TAACGCA17200.020.4593664
GGTATCA547200.020.3700561
TACACGG27600.019.7505462
GTATCAA749500.019.6691471
GTACACG33600.018.641761
GAGTACT515750.017.52494412
CTGGGGG127300.017.1278575
AGAGTAC797050.017.12669611
AGTACTT528800.017.09275413
GTACATG483100.016.9411051
TATCAAC889200.016.4628372
Produced by FastQC (version 0.11.5)