FastQCFastQC Report
Thu 26 May 2016
ERR488988_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488988_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18724415
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255770.13659705790541385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG263200.026.5417822
GTACCTG270800.026.277251
GTATCAA958400.025.5593151
GGTATCA611550.024.8087441
ACCTGGG302350.022.4268513
GAGTACT619900.020.99670412
TATCAAC1165050.020.818842
AGTACTT628400.020.72517613
TCAACGC1173000.020.7010774
ATCAACG1181150.020.5551473
CAACGCA1190450.020.3929545
GTACATG946900.019.9899121
AACGCAG1226100.019.8810566
GTACTTT654100.019.79042214
TACATGG961900.019.3762442
ACATGGG989300.018.3125293
AGAGTAC1250400.018.21392811
TACTTTT714500.018.11982715
ACTTTTT736550.018.00357816
TATCACG10600.017.6091162
Produced by FastQC (version 0.11.5)