FastQCFastQC Report
Thu 26 May 2016
ERR488988_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488988_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18724415
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240740.1285701048604189No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA606650.025.4867651
TACCTGG258100.025.236962
GTACCTG268050.024.704081
GTATCAA928450.024.605111
ACCTGGG293850.021.027983
TATCAAC1146450.019.8554732
GAGTACT626850.019.6693312
TCAACGC1153300.019.641244
ATCAACG1156950.019.5591853
CAACGCA1171800.019.3075455
AGTACTT637100.019.28233313
GTACATG947800.019.1165031
AACGCAG1208400.018.8085586
GTACTTT653000.018.67018314
TACATGG964300.018.5542492
TACCCGG33700.017.9155942
ACATGGG991550.017.581763
TACTTTT710800.017.1809215
ACTTTTT723000.016.98643716
AGAGTAC1271150.016.98118811
Produced by FastQC (version 0.11.5)