FastQCFastQC Report
Thu 26 May 2016
ERR488987_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488987_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9177922
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA1599711.7429980337597115No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA427330.46560648477945227No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA334440.3643962108198348No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC270370.29458738045496574No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149650.163054338443931No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG129440.1410341033623951No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126090.13738403965516377No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96110.10471869340358307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG219450.045.2363321
TACCTGG220000.044.7425542
ACCTGGG235000.041.181933
CCTGGGG179650.035.33514
TATAACG10900.034.5017932
GAGTACG29050.033.339031
ATAACGC12500.032.8457073
TACGCAG30500.029.8620594
TACCCGG19850.029.721952
GTACCCG20950.029.0582681
TAACGCA13600.028.918894
ACGCAGT32150.028.2221725
GTACGCA32250.028.1363493
TACACGG17350.026.6469212
CTGGGGG92600.026.4895325
AGTACGC34750.026.3107222
GTACACG19550.025.47751
TACCGGG13600.024.861592
GTACCGG16850.023.812181
CGCAGTG38900.023.5023846
Produced by FastQC (version 0.11.5)