FastQCFastQC Report
Thu 26 May 2016
ERR488986_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488986_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19494813
Sequences flagged as poor quality0
Sequence length75
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370110.189850500233062No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255590.13110666924581427No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG279850.026.2610052
GTACCTG295600.025.38181
ACCTGGG319150.022.2117253
GAGTACT685050.020.86004412
TATAACG14200.020.6920722
AGTACTT694600.020.5688113
GTATCAA1426000.019.6316721
GTACATG1042100.019.607621
GTACTTT730400.019.49178914
TACATGG1065450.018.8664442
AGAGTAC1324650.018.83067711
TACTTTT768800.018.5690415
ACATGGG1096800.017.8802413
ACTTTTT812900.017.8785816
GTATAGG46300.017.2583121
GTACCCG36600.017.1066441
GGTATCA1047300.016.6565531
TATCAAC1682700.016.5516032
TCAACGC1689000.016.482614
CCTGGGG260000.016.4166184
Produced by FastQC (version 0.11.5)