FastQCFastQC Report
Thu 26 May 2016
ERR488986_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488986_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19494813
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT359400.18435673119819104No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263920.13537960071738056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG278700.025.7331712
GTACCTG289600.025.2296141
ACCTGGG318500.021.6083433
GAGTACT709600.019.64825212
AGTACTT716650.019.36351413
TATAACG15050.019.0246812
GTATCAA1465050.018.634141
GTACTTT747550.018.50313614
GTACATG1058100.018.2746941
AGAGTAC1382300.017.95684811
TACTTTT782300.017.67222215
TACATGG1081600.017.615062
GGTATCA1094100.017.3921051
ACTTTTT818650.017.0055116
ACATGGG1101900.016.7087153
CCTGGGG252350.016.3361344
GTACCCG34400.016.1836741
TCAACGC1737000.015.6061914
TATCAAC1744050.015.5764112
ATCAACG1743200.015.5329933
Produced by FastQC (version 0.11.5)