FastQCFastQC Report
Thu 26 May 2016
ERR488985_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488985_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10879343
Sequences flagged as poor quality0
Sequence length75
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA1040470.9563720897484344No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280890.25818654674275826No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA265730.24425188175425666No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249120.22898441569495512No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG188610.17336524825074454No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178020.1636312045681435No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135970.12497997351494479No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126200.11599965181720992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG268450.047.3256232
GTACCTG275100.046.512931
ACCTGGG299350.041.660743
TATAACG11050.036.613752
CCTGGGG214950.036.5134244
ATAACGC13450.031.3615593
TACCCGG24250.030.5156522
CTGGGGG123900.027.783885
TAACGCA16650.027.6185934
GTACCCG30500.025.9726141
TATCACG8500.024.8159872
GTACACG23250.024.5494041
TACACGG23100.022.9034082
ATATCTA28300.022.7356051
TACCGGG16950.022.2370622
GAGTACG23400.022.1745781
TATCTAA28700.022.0490462
CCCGTAA16050.021.9260654
GTACCGG19350.021.6313881
GTATAAC24350.021.4515151
Produced by FastQC (version 0.11.5)