FastQCFastQC Report
Thu 26 May 2016
ERR488985_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488985_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10879343
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA1106571.0171294351138667No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250100.22988520538418541No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231310.21261394185292257No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG212710.1955173212205921No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC190600.17519440282377344No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188400.17317222188876663No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA176370.16211456886688838No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145640.13386837789745207No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126970.11670741514446231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG274500.045.463162
GTACCTG279300.045.021
ACCTGGG300150.040.7850273
TATAACG13550.038.4495352
CCTGGGG217150.035.319224
ATAACGC15850.034.1763653
TACCCGG23300.029.912242
TAACGCA17650.029.7118954
CTGGGGG119100.027.9826555
CCCGTAA16050.025.36264454
GTACCCG29650.025.2139031
TATCACG8000.024.151912
ACCCGTA17350.024.05877353
GTATAAC26450.022.8993641
TACACGG20350.022.888792
CGTAATG26950.022.65698256
ATATCTA27400.022.6106761
GTACACG22450.022.0460471
GAGTACG22350.021.8349721
TATCTAA29250.021.3504092
Produced by FastQC (version 0.11.5)