FastQCFastQC Report
Thu 26 May 2016
ERR488984_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488984_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15174841
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA1127330.7428941100601977No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268910.1772077875478234No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201400.13271967726053935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG178450.022.0107211
TACCTGG174050.021.9788632
GAGTACT542950.021.51184712
AGTACTT552150.021.06705313
GTACTTT570050.020.33261914
AGAGTAC1056950.018.95348711
TACTTTT616550.018.83157515
ACTTTTT636550.018.5123816
ACCTGGG197900.018.418123
GTACATG764600.018.0863381
TACATGG776350.017.5495572
TATAACG11450.017.249622
GAGTACG20500.017.2491321
ACATGGG796600.016.5844763
GTATAGG28600.015.5154681
CATGGGG460550.015.1821814
CTTTTTT792200.014.99563917
GTATATA41400.014.9890321
CCTGGGG164650.013.973214
CATGGGT113350.013.9696184
Produced by FastQC (version 0.11.5)