FastQCFastQC Report
Thu 26 May 2016
ERR488984_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488984_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15174841
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA1166870.7689503962512688No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA357670.2356993394527165No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA295680.19484882905857137No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG272740.17973170196643248No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263510.173649265913231No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207520.13675266844641074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG183200.022.4126761
TACCTGG181600.022.0947342
GAGTACT570150.020.2150612
AGTACTT577600.019.86473313
GTACTTT594600.019.15754514
ACCTGGG206800.018.6849693
AGAGTAC1109600.017.8767111
TACTTTT638400.017.79423715
GTACATG773750.017.4443631
ACTTTTT659350.017.25500716
TACATGG781500.017.0096322
TATAACG12500.016.5602232
ACATGGG797100.016.1876453
GAGTACG22250.016.1685751
CATGGGG465550.014.6509814
TAACGCA14450.014.5642574
CTTTTTT811950.014.10109317
GTATAGG30550.013.9271531
GTGTAGC74050.013.8272291
CCTGGGG166350.013.6467564
Produced by FastQC (version 0.11.5)