FastQCFastQC Report
Thu 26 May 2016
ERR488983_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488983_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10401674
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA528270.507870175512134No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184400.17727915718181517No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136100.130844323711741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG126700.025.7565961
TACCTGG127200.024.7005442
ACCTGGG140400.021.2931943
GAGTACT371350.020.08846712
AGTACTT378300.019.71163213
AGAGTAC609200.018.70641911
GTACTTT396000.018.66363514
GTACATG374550.018.4684471
TACATGG382000.017.7798862
CGCGTTC9050.017.53936260
TACTTTT426000.017.33395215
ACTTTTT442450.017.07176816
ACATGGG396900.016.7364773
CCTGGGG114200.015.3121484
ACGTCGT6150.015.13845523
GAGTACG14000.014.8154991
CATGGGG223700.014.6606064
GTGTAGC50300.014.4325931
ATAACGC10300.014.0930133
GTATAGA26100.014.039721
Produced by FastQC (version 0.11.5)