FastQCFastQC Report
Thu 26 May 2016
ERR488983_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488983_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10401674
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA189690.18236487703806137No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185790.1786154805466889No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151550.14569770211987032No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110140.10588680245122084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG122900.023.507111
TACCTGG124800.022.695342
ACCTGGG134250.020.4296513
GAGTACT432850.017.97234312
TATCACG5000.017.9395472
AGTACTT438850.017.648113
GTACTTT456550.016.88833614
GAGTACG16800.016.6817041
AGAGTAC721950.015.96955511
GTATAGG20850.015.9305141
TACTTTT485700.015.8959915
ACTTTTT505250.015.43795816
GTACATG404950.015.1742141
TATAACG10100.015.0293232
ATAACGC12400.014.7455953
TACCCGG15500.014.6899522
TACATGG414050.014.6228652
TACGCAG14700.014.55065254
CCTGGGG113450.014.0490064
TAACGCA11750.013.7996514
Produced by FastQC (version 0.11.5)