FastQCFastQC Report
Thu 26 May 2016
ERR488982_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488982_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13958181
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255010.18269572518080973No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA232260.16639703984351542No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184380.13209457593364063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA800200.024.1977181
GGTATCA530050.022.9583191
GTACCTG152450.021.9684541
TACCTGG146900.021.7485282
GAGTACT520950.021.34100212
AGTACTT531800.020.8355713
TATCAAC957450.020.1296232
ATCAACG964500.019.9402123
TCAACGC965950.019.8785784
GTACTTT556500.019.7863214
AGAGTAC905350.019.75795611
CAACGCA980450.019.5398395
AACGCAG1006900.019.141966
GTACATG662300.018.8345931
TACTTTT596250.018.508815
TACATGG665550.018.4151462
ACTTTTT626300.017.89233816
TATAACG9750.017.7699492
ACCTGGG172300.017.6349853
ACATGGG682450.017.4083423
Produced by FastQC (version 0.11.5)