FastQCFastQC Report
Thu 26 May 2016
ERR488982_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488982_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13958181
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250610.17954345197271765No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195320.13993227341012413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA837650.022.3041841
GGTATCA575650.021.9395681
TACCTGG149250.021.0361392
GTACCTG152000.020.9599761
GAGTACT533050.019.99149912
AGTACTT541700.019.64679713
GTACTTT561150.018.79368214
GTACATG685100.018.7274061
AGAGTAC933300.018.65176211
TATCAAC1006050.018.4948062
ATCAACG1004450.018.4177883
TCAACGC1007750.018.3746624
TACATGG689600.018.211392
CAACGCA1023900.018.0915765
AACGCAG1047550.017.7127746
ACCTGGG168200.017.4969373
TACTTTT601300.017.45223215
ACATGGG703250.017.3869323
ACTTTTT628450.016.86294616
TATAACG9650.016.8038772
Produced by FastQC (version 0.11.5)