FastQCFastQC Report
Thu 26 May 2016
ERR488981_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488981_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15743101
Sequences flagged as poor quality0
Sequence length75
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255220.16211545616076528No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181050.11500275581030701No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA322000.038.0692631
GTATCAA587300.031.8197671
TACCTGG225750.026.444952
GTACCTG230900.026.4017811
TATAACG10950.025.8867722
TCAACGC763650.024.3169144
TATCAAC766700.024.189252
ATCAACG767650.024.174763
CAACGCA779650.023.7928925
AACGCAG810300.022.968166
ACCTGGG265050.021.8392733
GAGTACT531700.020.12374912
GTACATG717800.020.0036721
AGTACTT539050.019.86995113
ACGCAGA932850.019.8371967
TACCCGG30350.019.5906092
CGCAGAG949300.019.4769748
GTACCCG32300.019.346511
TACATGG734600.019.1711542
AGAGTAC940300.019.04259711
Produced by FastQC (version 0.11.5)