FastQCFastQC Report
Thu 26 May 2016
ERR488981_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488981_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15743101
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260760.16563445791270728No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191290.12150719226154999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA330050.038.5454751
GTATCAA595100.031.468041
TACCTGG228850.027.5434572
GTACCTG235300.027.2833371
TCAACGC777750.023.9715734
TATCAAC784050.023.867492
ATCAACG782950.023.81733
CAACGCA791350.023.55965
ACCTGGG263650.023.0049843
AACGCAG821100.022.7942286
TATAACG10650.021.0569082
GAGTACT546750.020.08238212
ACGCAGA948050.019.7070527
AGTACTT557200.019.68098413
CGCAGAG968250.019.2959188
GTACATG714300.018.9628431
GTACTTT574700.018.96163614
AGAGTAC966750.018.92233811
TACATGG730000.018.2760412
CAGAGTA1025800.018.06534410
Produced by FastQC (version 0.11.5)