FastQCFastQC Report
Thu 26 May 2016
ERR488980_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488980_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13833869
Sequences flagged as poor quality0
Sequence length75
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA888990.6426184894478906No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281600.2035583826910606No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198200.14327156054463144No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142210.1027984289861354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG286450.042.5158352
GTACCTG291950.042.2532231
ACCTGGG317150.037.849793
CCTGGGG243150.032.765034
TATAACG12150.031.2970432
GTACACG34750.023.7719551
CTGGGGG150050.023.603935
GTACCCG29500.023.550271
ATAACGC16200.023.469353
TACCCGG28600.023.0862942
TACACGG29150.022.4135232
TAACGCA18250.021.4007744
GTACATG497800.020.8230061
TACATGG510300.020.058562
GAGTACT476150.019.79926912
AGTACTT485400.019.35894613
TATCACG10250.019.223742
AGAGTAC753600.019.06654511
ACATGGG521750.018.9331653
TGGGGGG217150.018.5845646
Produced by FastQC (version 0.11.5)