FastQCFastQC Report
Thu 26 May 2016
ERR488980_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488980_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13833869
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266930.19295397404731823No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202910.14667624798239742No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150080.10848736532057662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG290550.041.370722
GTACCTG298950.040.596631
ACCTGGG315200.037.2822463
CCTGGGG239800.031.306054
TATAACG14050.031.1862532
TAACGCA17500.025.8256824
ATAACGC17750.025.6577913
TACACGG25900.023.7113382
GTACACG31500.023.0641651
CTGGGGG140550.022.4844155
GTACCCG26350.021.9262731
TACCCGG26650.020.454822
TATCACG11850.019.215932
GTATAAC30200.019.1310371
GTACATG483550.018.8810861
GAGTACG24450.018.536231
TGGGGGG196900.018.1701896
TACATGG499650.018.1258852
GAGTACT503650.017.52867112
TACCGGG17000.017.4536132
Produced by FastQC (version 0.11.5)