FastQCFastQC Report
Thu 26 May 2016
ERR488979_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488979_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13924931
Sequences flagged as poor quality0
Sequence length75
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA465470.33427095617206287No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213530.15334366827383203No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155170.11143322720952802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG160200.024.544211
TACCTGG159700.024.1197592
GAGTACT447200.019.71348412
AGTACTT456400.019.33988813
GTACATG479250.019.1004221
AGAGTAC711550.018.78886811
ACCTGGG200100.018.781123
GTACTTT476600.018.40317714
TACATGG497750.018.0545942
TACTTTT510100.017.19470215
ACATGGG510350.016.9834233
TATAACG8400.016.8770792
ACTTTTT531750.016.83759316
GTATCAA963200.016.1564961
GTACCCG23400.015.2220271
CATGGGG320100.014.8601914
GTGTAGC70200.014.7294051
TACCCGG23550.014.682562
TATCAAC1080700.014.2731492
TCAACGC1089400.014.1209634
Produced by FastQC (version 0.11.5)