FastQCFastQC Report
Thu 26 May 2016
ERR488979_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488979_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13924931
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205350.1474693124152644No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161810.11620165299203278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG161150.024.3453711
TACCTGG166200.023.1242662
ACCTGGG206000.018.1541463
GTACATG486900.017.8576281
GAGTACT449350.017.80360612
AGTACTT457200.017.4677413
TATAACG8500.017.4527282
AGAGTAC723200.017.26800211
TACATGG505550.016.8420452
GTACTTT471000.016.7801614
ACATGGG511800.016.0768853
TACTTTT502050.015.7833715
ACTTTTT520500.015.39622316
GTATCAA944450.014.98450851
GGTATCA753250.014.3243571
ATAACGC13500.014.3109363
CCTGGGG172400.014.1679894
CATGGGG321150.013.718144
TATCACG6600.013.5907422
GAGTACA389150.013.1336711
Produced by FastQC (version 0.11.5)