FastQCFastQC Report
Thu 26 May 2016
ERR488978_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488978_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11163281
Sequences flagged as poor quality0
Sequence length75
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG332700.2980306596241732No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC258130.23123130198012573No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244700.2192007887286901No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175390.1571133074586226No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118090.1057843119778137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG21900.025.523641
GTACCTG160600.023.4555171
TACCTGG157900.023.3353752
ACCTGGG152800.022.816663
GAGTACT460000.021.3855412
GTACCCG14900.021.203911
TACCCGG14750.021.0906622
TACGCAG23350.021.0111734
AGTACTT469650.020.93992213
GTACTTT483850.020.12546514
TACTTTT513750.018.95531515
GTACATG773900.018.9271621
TACACGG16850.018.8724232
ACTTTTT527700.018.64248516
TACATGG774800.018.6299042
TATAACG8050.018.4634252
ACGCAGT26400.018.188255
ACATGGG776550.017.989483
AGAGTAC1009550.017.58180411
CATGGGT117200.017.304514
Produced by FastQC (version 0.11.5)