FastQCFastQC Report
Thu 26 May 2016
ERR488978_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488978_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11163281
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA1598411.431846067477832No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA476420.42677417150029634No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242450.21718525225693058No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183690.16454839755444658No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122000.10928686646873799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG166250.023.9513381
TACCTGG163600.023.874042
ACCTGGG159050.023.3204843
GAGTACT475750.020.78864112
GAGTACG20450.020.4695451
AGTACTT483650.020.39914513
GTACTTT499400.019.63837214
TACCCGG13100.019.2271232
GTACATG756150.018.6072561
TACTTTT531450.018.46669815
TACATGG761200.018.190022
ACTTTTT545900.018.04083316
GTACCCG14750.017.8253351
ACATGGG764750.017.555153
GTATAGG29400.017.5329531
TAGGGCG10750.017.32801631
CCTGGGG112750.016.8608074
AGAGTAC1016100.016.72720111
CATGGGT119250.016.5204184
CATGGGG393700.016.2650684
Produced by FastQC (version 0.11.5)