FastQCFastQC Report
Thu 26 May 2016
ERR488977_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488977_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17861831
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA1267660.7097032773403802No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT331060.18534494028075846No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA282840.15834882773216252No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225850.12644280421195342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG299000.035.167551
TACCTGG299250.034.8099022
ACCTGGG330450.030.8181553
TATAACG15350.023.8666342
CCTGGGG268900.023.4533214
TACACGG33150.020.5388622
GAGTACT594350.020.02966712
GTACCCG40150.019.6227861
AGTACTT607100.019.5871313
AGAGTAC994200.019.45999311
ATAACGC20350.019.3585433
TATCACG12900.019.0222722
TACCCGG40850.018.3595242
GTACACG43700.018.3450091
GTACTTT649950.018.20642914
GTACATG641950.018.1777971
GTATAAC32050.017.5740131
TACATGG659200.017.3908982
TACTTTT691900.017.14538415
TACCGGG22750.017.0149382
Produced by FastQC (version 0.11.5)