FastQCFastQC Report
Thu 26 May 2016
ERR488977_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488977_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17861831
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA1308870.7327748202297961No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA368920.20654097555844078No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT312820.17513322122463257No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA308730.17284342237926223No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238020.1332562154462216No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC231900.12982991497344254No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA225140.12604530856886956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG296000.033.514641
TACCTGG296300.032.9862062
ACCTGGG330100.029.0966873
TATAACG15950.023.1441122
CCTGGGG271550.022.2581754
ATAACGC20000.020.1825353
TACACGG34350.019.0828952
GAGTACT616150.018.63927512
TACCCGG38000.018.520982
AGTACTT625550.018.29285213
GTACCCG40800.018.142651
AGAGTAC1041750.017.51170211
TAACGCA22150.017.444144
TACCGGG22000.017.2499352
GTACTTT663100.017.22054714
GTACATG659200.017.1899191
GTACACG44550.017.0813471
GTATAAC34300.017.0427511
GAGTACG34700.016.5472471
TACATGG676750.016.3896662
Produced by FastQC (version 0.11.5)