FastQCFastQC Report
Thu 26 May 2016
ERR488976_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488976_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19229600
Sequences flagged as poor quality0
Sequence length75
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA13368816.952203893996756No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA3898012.0270884469775763No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA2972451.5457679826933477No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG2747051.4285528560136456No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC2237631.163638349211632No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC2174701.1309127594957773No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG1110000.5772350958938304No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA449400.2337022090943129No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC399390.2076954278820152No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGTAA236470.12297187669010276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTATG81850.033.774311
GAGTACG133500.031.5552521
TACGCAG141700.029.3196434
CTTATGT97750.028.0988942
ACGCAGT149600.027.687635
AGTACGC159400.026.3243712
CGCAGTG159150.026.118016
TTATGTA106750.025.5978573
GTACGCA165500.025.4144083
GTACCTG155950.024.8748191
TACCTGG153200.024.815582
TATGTAC118500.022.9450874
ACCTGGG171900.021.3694363
GAGTACT360600.018.64385812
AGTACTT363950.018.41637813
AGTAGGC68000.017.7542382
GAGTAGG75200.017.7339521
GTACATG658300.017.6309091
GTACTTT381300.017.42494614
TACATGG668600.017.1049562
Produced by FastQC (version 0.11.5)