FastQCFastQC Report
Thu 26 May 2016
ERR488975_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488975_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25286639
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG288050.023.9044061
TACCTGG284850.023.6587542
GAGTACT705650.020.40451812
AGAGTAC1312800.019.96751811
AGTACTT722800.019.78834313
GTATCAA1522750.019.3956281
GTACTTT746050.019.2485214
ACCTGGG350200.018.6985513
TACTTTT797900.017.91096715
GTACATG996700.017.5921151
TACATGG1007050.017.095722
ACTTTTT860450.016.92622416
TATCAAC1752200.016.7027592
TCAACGC1760800.016.5784874
ATCAACG1763650.016.5492023
TATAACG15600.016.402252
CAACGCA1785700.016.3352225
AACGCAG1832550.016.0395456
GGTATCA1126150.016.0367071
ACATGGG1049000.015.9025193
Produced by FastQC (version 0.11.5)