FastQCFastQC Report
Thu 26 May 2016
ERR488975_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488975_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25286639
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG299650.022.892141
TACCTGG294150.022.6945672
GAGTACT716950.018.82822212
ACCTGGG352500.018.487693
AGTACTT733500.018.30011213
AGAGTAC1346450.018.2630611
GTATCAA1523300.018.0375541
GTACTTT751550.017.74125114
GTACATG1011500.016.8976141
TATAACG17150.016.6964362
TACTTTT810650.016.4689515
TACATGG1029750.016.3056982
GGTATCA1158700.015.98221
ACTTTTT854850.015.71831116
TATCAAC1768850.015.4333432
TCAACGC1770400.015.3438314
ATCAACG1772850.015.3031673
ACATGGG1060300.015.2534693
ATAACGC21500.015.2438593
CAACGCA1797850.015.1097375
Produced by FastQC (version 0.11.5)