FastQCFastQC Report
Thu 26 May 2016
ERR488974_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488974_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9223957
Sequences flagged as poor quality0
Sequence length75
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA921150.9986494950052348No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT388640.42133761031193007No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294600.3193857039879956No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA246520.2672605694063838No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA213490.23145164271689472No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194290.2106362811535223No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124460.13493124480090268No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102020.11060329097371116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTGGG113900.029.0365963
TACCTGG121550.027.9485022
GTACCTG130350.026.7032071
TACCCGG10400.024.2823942
CCTGGGG96000.023.713974
CTGGGGG70350.022.8583855
GTACCCG13100.022.2748471
TATAACG7050.021.5906412
GAGTACT463500.021.22887212
TATCACG5400.021.1408372
AGTACTT466900.021.08962813
GTACATG652350.020.8689861
TACATGG661450.020.3971542
GTACACG16050.020.3450891
TGGGGGG371400.020.0829056
GTACTTT493950.019.89369614
ACATGGG665100.019.8071273
GAGTACG12500.019.7312741
ATGGGGG363300.019.4949115
CATGGGG465800.019.2524134
Produced by FastQC (version 0.11.5)