FastQCFastQC Report
Thu 26 May 2016
ERR488974_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488974_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9223957
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA927841.0059023475499722No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT377780.4095639214276476No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302380.3278202619548205No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA237110.25705887397350186No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205180.2224424940402476No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126500.137142876967011No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97930.10616918530734695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTGGG111400.028.030493
TACCTGG120200.026.8395042
GTACCTG128950.025.9184571
CCTGGGG93350.023.2116284
GAGTACG12150.022.7806991
TACCGGG6100.022.0598282
CTGGGGG69600.022.010715
TATAACG6850.021.659342
TATCACG4950.021.6084612
GAGTACT464900.020.19202212
AGTACTT467900.020.1289213
GTACATG657050.019.772651
TACATGG666450.019.3732992
GTACTTT492800.018.92283414
TGGGGGG353600.018.890936
ACATGGG668300.018.829193
GTACACG14100.018.4032921
CATGGGG459500.018.2390544
AGAGTAC872000.017.91433711
ATGGGGG355600.017.9017265
Produced by FastQC (version 0.11.5)